Nursing and clinical course on a burned person complicated by polymicrobial infections

Here we describe the assistential complexity in an Internal Medicine Division via a multidisciplinary approach taking care of a burned woman. She was transferred in our Division after 90 days of hospitalisation in an Intensive Care Unit from an important centre for burned people for the treatment of burns on 35-40% of the body surface area. We analyse the therapeutic strategies and nursing management in a case complicated by polymicrobial infections: Acinetobacter baumanii and Providencia stuartii on burns, Escherichia coli on respiratory system and Clostridium difficile on enteric apparatus

Post‐budburst hand finishing of winter spur pruning can delay technological ripening without altering phenolic maturity of Merlot berries

Background and Aims Global warming can lead to technological ripening occurring in advance of phenolic maturity for red wine cultivars. This study evaluated the effect of post‐budburst winter pruning on the phenology, yield components, berry composition and phenolic maturity in Vitis vinifera L. cv. Merlot. Methods and Results Mechanically pre‐pruned vines followed up with hand pruning during winter (Control) were compared to vines that were mechanically pre‐pruned and followed up with hand pruning after budburst when distal buds developed shoots with either three unfolded leaves (DF3) or eight unfolded leaves (DF8). Late pruning delayed budburst, flowering and, to a lesser extent, veraison. The delays were greater for DF8 than DF3 treatments. Yield decreased by about 40 and 71% in DF3 and DF8 vines, respectively, while sugar accumulation and reduction of TA were delayed by both pruning treatments. The concentration of anthocyanin and tannin and of extractable anthocyanin and tannin (of skin and seeds analysed separately) were not influenced by the DF3 treatment while tannin concentration increased in DF8 berries. Conclusions Delaying hand pruning of mechanically pre‐pruned vines until after budburst of distal nodes can delay technological ripening without affecting the concentration of anthocyanin and tannin of berries. Yield, however, is substantially reduced. Significance of the Study We verified the feasibility of a cost‐effective technique that can be adopted to counteract the hastening of sugar accumulation and organic acid decline caused by global warming on valuable black grapes

Beyond multi-view deconvolution for inherently-aligned fluorescence tomography

In multi-view fluorescence microscopy, each angular acquisition needs to be aligned with care to obtain an optimal volumetric reconstruction. Here, instead, we propose a neat protocol based on auto-correlation inversion, that leads directly to the formation of inherently aligned tomographies. Our method generates sharp reconstructions, with the same accuracy reachable after sub-pixel alignment but with improved point-spread-function. The procedure can be performed simultaneously with deconvolution further increasing the reconstruction resolution

Gingival reactive lesions in orally rehabilitated patients by free revascularized flap

The aim is to discuss four cases of gingival reactive hyperplastic lesions in patients with a history of excision of oral neoplastic lesions and rehabilitation by a free revascularized flap of the iliac crest. One female and 3 male patients were referred due to the presence of exophytic lesions at the rehabilitated sites. The clinical examination revealed that the poor oral hygiene was the common trigger factor in all the cases, in addition to trauma from the upper left second molar in the first case, pericoronitis related to a partially erupted lower right third molar in the third case, and poor stability of an upper removable partial denture in the fourth case. All the cases were subjected to elimination of these suspected triggering factors, exclusion of dysplasia, excisional biopsy by CO2 laser, and five follow-up visits. The histological examination of all the cases confirmed the diagnosis of pyogenic granuloma. These presented cases suggest that the limitations in oral functions and maintaining the oral hygiene measures following the free revascularized flap reconstruction surgery probably played a role in the development of gingival reactive hyperplastic lesions with presence of trigger factors such as local trauma, chronic infection, or inadequate prosthesis

A photoluminescence study of the changes induced in the zinc white pigment by formation of zinc complexes

It is known that oil paintings containing zinc white are subject to rapid degradation. This is caused by the interaction between the active groups of binder and the metal ions of the pigment, which gives rise to the formation of new zinc complexes (metal soaps). Ongoing studies on zinc white paints have been limited to the chemical mechanisms that lead to the formation of zinc complexes. On the contrary, little is known of the photo-physical changes induced in the zinc oxide crystal structure following this interaction. Time-resolved photoluminescence spectroscopy has been applied to follow modifications in the luminescent zinc white pigment when mixed with binder. Significant changes in trap state photoluminescence emissions have been detected: the enhancement of a blue emission combined with a change of the decay kinetic of the well-known green emission. Complementary data from molecular analysis of paints using Fourier transform infrared spectroscopy confirms the formation of zinc carboxylates and corroborates the mechanism for zinc complexes formation. We support the hypothesis that zinc ions migrate into binder creating novel vacancies, affecting the photoluminescence intensity and lifetime properties of zinc oxide. Here, we further demonstrate the advantages of a time-resolved photoluminescence approach for studying defects in semiconductor pigments

Autonomous Observations in Antarctica with AMICA

The Antarctic Multiband Infrared Camera (AMICA) is a double channel camera operating in the 2-28 micron infrared domain (KLMNQ bands) that will allow to characterize and exploit the exceptional advantages for Astronomy, expected from Dome C in Antarctica. The development of the camera control system is at its final stage. After the investigation of appropriate solutions against the critical environment, a reliable instrumentation has been developed. It is currently being integrated and tested to ensure the correct execution of automatic operations. Once it will be mounted on the International Robotic Antarctic Infrared Telescope (IRAIT), AMICA and its equipment will contribute to the accomplishment of a fully autonomous observatory.Comment: 12 pages, 4 figures, Advances in Astronomy Journal, Special Issue "Robotic Astronomy", Accepted 11 February 201

Detection of internal quality in kiwi with time-domain diffuse reflectance spectroscopy

Time-domain diffuse reflectance spectroscopy (TRS), a medical sensing technique, was used to evaluate internal kiwi fruit quality. The application of this pulsed laser spectroscopic technique was studied as a new, possible non-destructive, method to detect optically different quality parameters: firmness, sugar content, and acidity. The main difference with other spectroscopic techniques is that TRS estimates separately and at the same time absorbed light and scattering inside the sample, at each wavelength, allowing simultaneous estimations of firmness and chemical contents. Standard tests (flesh puncture, compression with ball, .Brix, total acidity, skin color) have been used as references to build estimative models, using a multivariate statistical approach. Classification functions of the fruits into three groups achieved a performance of 75% correctly classified fruits for firmness, 60% for sugar content, and 97% for acidity. Results demonstrate good potential for this technique to be used in the development of new sensors for non-destructive quality assessment

Selection Models for the Internal Quality of Fruit, based on Time Domain Laser Reflectance Spectroscopy

Time domain laser reflectance spectroscopy (TRS) was applied for the first time to evaluate internal fruit quality. This technique, known in medicine-related knowledge areas, has not been used before in agricultural or food research. It allows the simultaneous measurement of two optical characteristics of the sample: light scattering inside the tissues and light absorption. Models to estimate non-destructively firmness, soluble solids and acid contents in tomato, apple, peach and nectarine were developed using sequential statistical techniques: principal component analysis, multiple stepwise linear regression, clustering and discriminant analysis. Consistent correlations were established between the two parameters measured with TRS, i.e. absorption and transport scattering coefficients, with chemical constituents (soluble solids and acids) and firmness, respectively. Classification models were created to sort fruits into three quality grades (‘low’, ‘medium’ and ‘high’), according to their firmness, soluble solids and acidity

Light Sheet Fluorescence Microscopy Quantifies Calcium Oscillations in Root Hairs of Arabidopsis thaliana

Calcium oscillations play a role in the regulation of the development of tip-growing plant cells. Using optical microscopy, calcium oscillations have been observed in a few systems (e.g. pollen tubes, fungal hyphae and algal rhizoids). High-resolution, non-phototoxic and rapid imaging methods are required to study the calcium oscillation in root hairs. We show that light sheet fluorescence microscopy is optimal to image growing root hairs of Arabidopsis thaliana and to follow their oscillatory tip-focused calcium gradient. We describe a protocol for performing live imaging of root hairs in seedlings expressing the cytosol-localized ratiometric calcium indicator Yellow Cameleon 3.6. Using this protocol, we measured the calcium gradient in a large number of root hairs. We characterized their calcium oscillations and correlated them with the rate of hair growth. The method was then used to screen the effect of auxin on the properties of the growing root hairs